Summary
Lingual epithelial cells, including those of the taste buds, are regularly replaced by proliferative stem cells. We found that integrin β1, a keratinocyte stem cell marker, was expressed at the basal layer and taste buds of adult mouse tongue epithelium. We purified and cultured integrin β1-positive cells (termed KT-1 cells), whose growth was stimulated by epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2). FGF-2 stimulation induced translocation of the FGF type I receptor (FGFR1) into nuclei, suggesting that the growth-stimulating effect of FGF-2 was mediated through FGFR1. EGF and FGF-2 also regulated cell surface expression of the neural cell adhesion molecule (N-CAM) in KT-1 cells. Anti-N-CAM antibody immunoreactivity was restricted to the gustatory epithelium and the nerves in the tongue epithelium, giving rise to the possibility that KT-1 may contain gustatory epithelial cells. KT-1 cells may thus be useful for analyzing the factors that regulate the growth and differentiation of lingual and gustatory epithelial cells in vitro.
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Ookura, T., Kawamoto, K., Tsuzaki, H. et al. Fibroblast and epidermal growth factors modulate proliferation and neural cell adhesion molecule expression in epithelial cells derived from the adult mouse tongue. In Vitro Cell.Dev.Biol.-Animal 38, 365–372 (2002). https://doi.org/10.1290/1071-2690(2002)038<0365:FAEGFM>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2002)038<0365:FAEGFM>2.0.CO;2